Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170514_m6pv TCTGTTGCTCAGCTGAGGGGTTCTGGCTCAGG(+CAGTGT)GTACCTCTCTAGGTTTTCCGGGATAGTAATT NGG 10 8 17 8 24 0 100 5 10
    240605170512_ivk8 TAGGCCTGTGCCATACCAAATATTCACCAATG(+CAGTGT)GTACCCTCTAGAGTAAAGCAAAGCTAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605170511_g3qo CAACTTTTATTAGCTTTGCTTTACTCTAGAGG(+CAGTGT)GTACCATTGGTGAATATTTGGTATGGCACAG NGG 10 8 17 8 24 0 100 5 10
    240605170509_jdj2 TTCCAGCCTGCGGGTCTTGGGCACTGAACTTG(+CAGTGT)GATCCGCAGGCTTGGCAGCAGGAGCAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240605170507_41nz AGCTTGTACCTGCTCCTGCTGCCAAGCCTGCG(+CAGTGT)GATCCAAGTTCAGTGCCCAAGACCCGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605170506_fp5b TTTTAACCAAACAGATATTAAAATACAGTCTG(+CAGTGT)GATCCAGGCCAACAACACATCAAGCAGACTG NGG 10 8 17 8 24 0 100 5 10
    240605170504_8lyr TTCTTCAGTCTGCTTGATGTGTTGTTGGCCTG(+CAGTGT)GATCCAGACTGTATTTTAATATCTGTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605170503_ezr9 GTTACATATGCATGCAAAGAAATTAACTCAAG(+CAGTGT)GTACCCTGGTGTGTTGGCTTTTCAAACTTGA NGG 10 8 17 8 24 0 100 5 10
    240605170501_zjew CCATTTCAAGTTTGAAAAGCCAACACACCAGG(+CAGTGT)GTACCTTGAGTTAATTTCTTTGCATGCATAT NGG 10 8 17 8 24 0 100 5 10
    240605170459_wa8i TACTGGGGCTGGTGAAATGAGGTGAATGACAG(+CAGTGT)GATCCGTTGGTAAAACAGCCTGGTTTTGATT NGG 10 8 17 8 24 0 100 5 10