Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170618_cj3r TTTTCCTTTTAATTAAAAAGGTATTTAAAATG(+CAGTGT)GATCCCTATAAAGACTTCTAACTGCTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240605170616_whcg ATTCCTTTTTAAATTAATATTATACAGAATAG(+CAGTGT)GATCCTCTGAAGTGGGCAATATTTTGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605170615_ywaf TCTTGGTAAACAAAATATTGCCCACTTCAGAG(+CAGTGT)GATCCTATTCTGTATAATATTAATTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605170614_b6ep CTTTCTATTGAGTTGTCCCTGCCACCCATAAG(+CAGTGT)GTACCAGGTGAGCCATTTCTTTTCATCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605170612_kjac GCGCATACAGATGAAAAGAAATGGCTCACCTG(+CAGTGT)GTACCTTATGGGTGGCAGGGACAACTCAATA NGG 10 8 17 8 24 0 100 5 10
    240605170610_kxrl AGTAGGTGACCCCATAGGTGTACCACCATAGG(+CAGTGT)GTACCTGCACCCACACCTGTGTACTCACAAA NGG 10 8 17 8 24 0 100 5 10
    240605170608_dg14 TTTAATTTGTGAGTACACAGGTGTGGGTGCAG(+CAGTGT)GTACCCTATGGTGGTACACCTATGGGGTCAC NGG 10 8 17 8 24 0 100 5 10
    240605170607_yc6y AGATAGTTTTCAGTCTTTGACTCTGATGAATG(+CAGTGT)GTACCACCGTGAGCATTCTTGTATATGCATG NGG 10 8 17 8 24 0 100 5 10
    240605170605_dld0 AGAAACATGCATATACAAGAATGCTCACGGTG(+CAGTGT)GTACCATTCATCAGAGTCAAAGACTGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605170604_3cs2 TTTAATATCGTGTGTCTCGGGAATGCAAGATG(+CAGTGT)GATCCCAAAGGTTTTACTGTTTCCCTGCCTT NGG 10 8 17 8 24 0 100 5 10