Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170603_h6gg GCTAAAAGGCAGGGAAACAGTAAAACCTTTGG(+CAGTGT)GATCCATCTTGCATTCCCGAGACACACGATA NGG 10 8 17 8 24 0 100 5 10
    240605170601_7wsy TGGGACAAGGATGAGCAAGCCCTGGGCAGGTG(+CAGTGT)GTACCGAGGCTTGCCAGGGGCGGCTCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605170559_j8u1 AGGGCCCACTGAGCCGCCCCTGGCAAGCCTCG(+CAGTGT)GTACCACCTGCCCAGGGCTTGCTCATCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605170558_iyqf ATGGGCTAGTGTCTTTTGAGAGGAAATCAGTG(+CAGTGT)GATCCCGGATTTGCTCCTGTTAATGAAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605170555_mypz CTTTAAAACTTCATTAACAGGAGCAAATCCGG(+CAGTGT)GATCCACTGATTTCCTCTCAAAAGACACTAG NGG 10 8 17 8 24 0 100 5 10
    240605170554_6tvu AGGAACATTTTAAAGTTCCAACTATGGGAAAG(+CAGTGT)GTACCTGATACGGGGTTTCCCTGCAAATTTA NGG 10 8 17 8 24 0 100 5 10
    240605170553_szhb ATGTCTAAATTTGCAGGGAAACCCCGTATCAG(+CAGTGT)GTACCTTTCCCATAGTTGGAACTTTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240605170551_zgoh CCTTGTGCTTAGCTTCCTGGGAGGAGTGAACG(+CAGTGT)GTACCTGCCCTTCTCAGTGCTCCTGTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240605170550_qit2 CGTGATACTCACAGGAGCACTGAGAAGGGCAG(+CAGTGT)GTACCGTTCACTCCTCCCAGGAAGCTAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605170549_hizb TAAAACACCATTTTGTTTGGTGCTAGAGATAG(+CAGTGT)GATCCAGGCTTTGTCCATGCCAGGCAGGCAC NGG 10 8 17 8 24 0 100 5 10