Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170547_9jmr GAGGAGTGCCTGCCTGGCATGGACAAAGCCTG(+CAGTGT)GATCCTATCTCTAGCACCAAACAAAATGGTG NGG 10 8 17 8 24 0 100 5 10
    240605170545_xpbj TGTTTTAAGATTTTGAAAGGGAATTGTTGGTG(+CAGTGT)GATCCATTTGATATTTAATTTTATTTTTTGG NGG 10 8 17 8 24 0 100 5 10
    240605170544_wfqn TTTAACCAAAAAATAAAATTAAATATCAAATG(+CAGTGT)GATCCACCAACAATTCCCTTTCAAAATCTTA NGG 10 8 17 8 24 0 100 5 10
    240605170542_2otm TGGGGCAGAGTTGCCCACTCCGACAGTCCTCG(+CAGTGT)GATCCCCGCGCCCTGGGCGCGGTTCACACCG NGG 10 8 17 8 24 0 100 5 10
    240605170540_zcc7 CCGGCCGGTGTGAACCGCGCCCAGGGCGCGGG(+CAGTGT)GATCCGAGGACTGTCGGAGTGGGCAACTCTG NGG 10 8 17 8 24 0 100 5 10
    240605170539_fftx TTATCTCTGCGTAGTCCAAAACAAAGTAAATG(+CAGTGT)GTACCGCTGTCGTGCTGCGAAGGTGAGAACG NGG 10 8 17 8 24 0 100 5 10
    240605170537_5y36 ACTAGCGTTCTCACCTTCGCAGCACGACAGCG(+CAGTGT)GTACCATTTACTTTGTTTTGGACTACGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605170536_4151 CTCCAAGCCAGCTGGGCCATCAGATGCTAATG(+CAGTGT)GTACCCAGAAATCCCTCACTTGTCATCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605170534_i37r AATCCACAGGATGACAAGTGAGGGATTTCTGG(+CAGTGT)GTACCATTAGCATCTGATGGCCCAGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605170533_u5qf AGGGTAAGGAAATCTACTTGCCCTACCTGTGG(+CAGTGT)GATCCAGGCCTGGTTAAAATGGTACTTGACT NGG 10 8 17 8 24 0 100 5 10