Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170318_224j GAGGGTCCCAGTACACAGGCTCGGTTGTGCCG(+CAGTGT)GATCCCAACAACATGGACAGTGGAGAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605170317_nhsl GTAAATATGCAAAAAGCGTTTCCTAATGGCTG(+CAGTGT)GATCCATGGTGTCTGGCCCAATGAAGTAAGA NGG 10 8 17 8 24 0 100 5 10
    240605170315_j401 CTTTCTCTTACTTCATTGGGCCAGACACCATG(+CAGTGT)GATCCAGCCATTAGGAAACGCTTTTTGCATA NGG 10 8 17 8 24 0 100 5 10
    240605170314_wjin AGACAGCTTTCCGGGTCCAGATATCTAATCTG(+CAGTGT)GATCCATGCTAACAGTACAGCACACAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605170312_0f65 AGCTACAGCTTGTGTGCTGTACTGTTAGCATG(+CAGTGT)GATCCAGATTAGATATCTGGACCCGGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605170311_1fdf TTCTGGCAACACCCCTCTGAAAAGTAGATTAG(+CAGTGT)GTACCAACTCGCAAGTAGAGTGGGATTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605170310_thdz GGGAAGAGGAATCCCACTCTACTTGCGAGTTG(+CAGTGT)GTACCTAATCTACTTTTCAGAGGGGTGTTGC NGG 10 8 17 8 24 0 100 5 10
    240605170308_7xyb GCACTGCCAGACTTACTTACTAAGCCTGCTGG(+CAGTGT)GATCCCAGGCCTGCCCTGGGACAGAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605170306_ntvt TTCTCGGCCTCTCTGTCCCAGGGCAGGCCTGG(+CAGTGT)GATCCCAGCAGGCTTAGTAAGTAAGTCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605170304_j83i AAAACTGTAGCTTACTCATCTTAGTAGGTTAG(+CAGTGT)GTACCAAAGTCCAGGCTGTTTATTTCTAACC NGG 10 8 17 8 24 0 100 5 10