Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170247_efjg AGCAGGGCCAACTCTTAAATCTCAGCCACCAG(+CAGTGT)GTACCACCTGAGCCGAGACAATAATGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605170245_1826 GGCACTCACTAGGAGGACCTGTCCACACTGGG(+CAGTGT)GATCCTGCAGGCCCTGGGTGGGGACAGCACC NGG 10 8 17 8 24 0 100 5 10
    240605170244_du3f GCCAGGGTGCTGTCCCCACCCAGGGCCTGCAG(+CAGTGT)GATCCCCAGTGTGGACAGGTCCTCCTAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605170242_ga6f TCACTCAGAGTGGGGGACCTGTCCATACTGCG(+CAGTGT)GATCCTGCAGGCTCTGGGTGGGGACAGCACC NGG 10 8 17 8 24 0 100 5 10
    240605170241_3ezb GCCAGGGTGCTGTCCCCACCCAGAGCCTGCAG(+CAGTGT)GATCCGCAGTATGGACAGGTCCCCCACTCTG NGG 10 8 17 8 24 0 100 5 10
    240605170239_nm32 TGAGTTTGCCAGGAGCCCTGGGGTGCAGGCAG(+CAGTGT)GATCCCTGCAGCCCCACAGCCAACTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605170238_4nff GAAGAAGGAAAAGTTGGCTGTGGGGCTGCAGG(+CAGTGT)GATCCTGCCTGCACCCCAGGGCTCCTGGCAA NGG 10 8 17 8 24 0 100 5 10
    240605170237_zv83 AGCCGTGGAACACGTCTCTTAGGGTGTGCTTG(+CAGTGT)GATCCAGAGCCCTGCAGGTCTCTGGGGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605170235_izyb AGTGACAAACCCCAGAGACCTGCAGGGCTCTG(+CAGTGT)GATCCAAGCACACCCTAAGAGACGTGTTCCA NGG 10 8 17 8 24 0 100 5 10
    240605170233_x2xd TCTCTCTATCCCTTAAAAAGAACTATCTCCAG(+CAGTGT)GTACCCAGTCCTGTTGTAGAAACCACCTGAT NGG 10 8 17 8 24 0 100 5 10