Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170201_u1xd ATTATTATAGTAGTTCTCTCCCACCTGTCAGG(+CAGTGT)GATCCCCACTGGATGCCTGAAACTGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240605170159_xjzp AAAGGATAACTTTTCTTTCTTTTGACTATGTG(+CAGTGT)GATCCTACATATTGAACTCAGATAGTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605170158_r8gy GCTAACCCTGACTATCTGAGTTCAATATGTAG(+CAGTGT)GATCCACATAGTCAAAAGAAAGAAAAGTTAT NGG 10 8 17 8 24 0 100 5 10
    240605170157_ckcc GGGGGGGGGCGGGGGTGGTGTGGTCTGAACAG(+CAGTGT)GATCCCACAGTAGCTCAGGCTGGCCTTGAAC NGG 10 8 17 8 24 0 100 5 10
    240605170155_hmqt AGAAAGTTCAAGGCCAGCCTGAGCTACTGTGG(+CAGTGT)GATCCTGTTCAGACCACACCACCCCCGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605170154_niah ATGGAATCTTATTCCAACACAACTTCCAAATG(+CAGTGT)GATCCTCTCCTCAAAATAATCTGGTATGAAG NGG 10 8 17 8 24 0 100 5 10
    240605170153_3tz3 AGTTTCTTCATACCAGATTATTTTGAGGAGAG(+CAGTGT)GATCCATTTGGAAGTTGTGTTGGAATAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605170151_ard7 AATACATTCAGACTTCAGCTCTCCAGAGGAAG(+CAGTGT)GTACCCAGATCAATTTTTAGGTGGGAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605170149_qxa1 CTGTCATTTTTCCCACCTAAAAATTGATCTGG(+CAGTGT)GTACCTTCCTCTGGAGAGCTGAAGTCTGAAT NGG 10 8 17 8 24 0 100 5 10
    240605170147_tg6s CAATTCGCTCCTTCTGTTCCCCACATTCCAGG(+CAGTGT)GATCCTTCGGAGCAGGTTCTGTACGTCCAGC NGG 10 8 17 8 24 0 100 5 10