Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170115_9kw0 GTCCTCTGGCCTGCCCCTCTGCCTCCTGGCAG(+CAGTGT)GTACCTATGTAGGGGGCTGGCTGAAGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605170114_9ozn CTCAAGGCCGGTGTGCTGTAAGTTAGGGTTTG(+CAGTGT)GATCCTCTCCCTCTCATTTGTATATTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605170113_tat5 TCGAGGAAACAATATACAAATGAGAGGGAGAG(+CAGTGT)GATCCAAACCCTAACTTACAGCACACCGGCC NGG 10 8 17 8 24 0 100 5 10
    240605170111_504x AGAGACGGTGGTCTCAACTACATGCACAAGTG(+CAGTGT)GATCCTAAAAAATGTATGGAGATTTTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605170110_is8i TTCCATTTAAAAAATCTCCATACATTTTTTAG(+CAGTGT)GATCCACTTGTGCATGTAGTTGAGACCACCG NGG 10 8 17 8 24 0 100 5 10
    240605170108_v55w TGGCTACTTTACGTGTACGTGAATGAGGCTGG(+CAGTGT)GTACCAGATGTAAAGGCTAAGGGAGAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605170107_6236 TGTAGTCTCCTCTCCCTTAGCCTTTACATCTG(+CAGTGT)GTACCCAGCCTCATTCACGTACACGTAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605170105_9ae2 TTAACTGGAGTTTAATTAATAAACTATTTAGG(+CAGTGT)GATCCACCACCAGTCATATAGACATATTAAA NGG 10 8 17 8 24 0 100 5 10
    240605170104_97h7 TTTGTTTTAATATGTCTATATGACTGGTGGTG(+CAGTGT)GATCCCTAAATAGTTTATTAATTAAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240605170102_e5pb CATGTAGTGGCCTGTGGAGGTCATAAACAGTG(+CAGTGT)GATCCCTTGGAGCTGGAGTCACAGTTGGCTA NGG 10 8 17 8 24 0 100 5 10