Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605170013_4bbn TCTGCACGACTCTGCCCAAAGCTGAAGGTGAG(+CAGTGT)GTACCAGCTTTTTGGAGAGACATCAGTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605170012_w264 GCCAGCATGCAAGGCTCTGGGGTCAGTATCTG(+CAGTGT)GTACCAGAGGTGGGGTGGTAGGGTATAAACT NGG 10 8 17 8 24 0 100 5 10
    240605170010_0erv CAGTTAGTTTATACCCTACCACCCCACCTCTG(+CAGTGT)GTACCAGATACTGACCCCAGAGCCTTGCATG NGG 10 8 17 8 24 0 100 5 10
    240605170008_9up5 TATTTGTAAAGACTGGGACCCTACCTGAAGGG(+CAGTGT)GATCCAACCACTCCACAGGAAGGGCTAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605170006_moev AAAATGTTTTAGCCCTTCCTGTGGAGTGGTTG(+CAGTGT)GATCCCCTTCAGGTAGGGTCCCAGTCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605170005_kiag GCAGCAGACTGGGACCCTGGGAGGGCTGGCAG(+CAGTGT)GATCCTGCTAGGGCCCTGCTTTACCATCTCT NGG 10 8 17 8 24 0 100 5 10
    240605170004_rtzi CTGAAAGAGATGGTAAAGCAGGGCCCTAGCAG(+CAGTGT)GATCCTGCCAGCCCTCCCAGGGTCCCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605170002_abgk AAAAGGATATACCTTCTTCTCAGCACCTCATG(+CAGTGT)GTACCTTCTCCAAAATTGACCACATAATAGG NGG 10 8 17 8 24 0 100 5 10
    240605170001_v3fj TGTGACCTATTATGTGGTCAATTTTGGAGAAG(+CAGTGT)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240605165959_dlpj ACGAAATGGACAAATTTCTGGACAGATACCAG(+CAGTGT)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10