Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165910_nniz ACAGATAGCTCCCCTGGATCTTATCAAAATAG(+CAGTGT)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605165909_cmpl GCTATATAGCATTGATCAAATACTTAAATAAG(+CAGTGT)GATCCCAAGCAAGATCTATAGATTGTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605165907_f0bq GTGCATCCCAACAATCTATAGATCTTGCTTGG(+CAGTGT)GATCCTTATTTAAGTATTTGATCAATGCTAT NGG 10 8 17 8 24 0 100 5 10
    240605165905_ipww TAATAAGTCTCGGTGTCATCATTTAACTGGAG(+CAGTGT)GATCCAGAAAGTCCCACTACAATGTATTGCA NGG 10 8 17 8 24 0 100 5 10
    240605165904_imm9 CTATCTGCAATACATTGTAGTGGGACTTTCTG(+CAGTGT)GATCCTCCAGTTAAATGATGACACCGAGACT NGG 10 8 17 8 24 0 100 5 10
    240605165903_a3ap GACAGATCCAGTTCAAAATCCTAGAGGCAGTG(+CAGTGT)GATCCTACAAGAGACTTGTGATTTTTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605165901_i7m8 AGGGAAGAAAAAAAATCACAAGTCTCTTGTAG(+CAGTGT)GATCCACTGCCTCTAGGATTTTGAACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605165900_6wx3 CCAGTGCTAGCACTTGCTTACTGCCTCAGCTG(+CAGTGT)GATCCTAACACTAGCCCTTCAAGATTAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605165858_mvj1 ATCCTGGCTTAATCTTGAAGGGCTAGTGTTAG(+CAGTGT)GATCCAGCTGAGGCAGTAAGCAAGTGCTAGC NGG 10 8 17 8 24 0 100 5 10
    240605165857_9mef TATTTTTTAAAAATTATTTTTGTGGACATGGG(+CAGTGT)GATCCCGTGACGGCACCTACTTTTGGGAGTC NGG 10 8 17 8 24 0 100 5 10