Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165958_d24p TAGAAGGTCAACTTGATCCTGATTCAACTTTG(+CAGTGT)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605165957_k7qm CATTAGGTAATCATTCCTGCCTTTAAAACAAG(+CAGTGT)GATCCTGCTGGCTCCTCTTTTCAGAGTCACT NGG 10 8 17 8 24 0 100 5 10
    240605165956_pjxu CTTACAGTGACTCTGAAAAGAGGAGCCAGCAG(+CAGTGT)GATCCTTGTTTTAAAGGCAGGAATGATTACC NGG 10 8 17 8 24 0 100 5 10
    240605165954_7gu7 GGTGTCAGCGTTTGGAAGCTGATTATGGGATG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605165952_q0yg GGATGGACCATCTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCATCCCATAATCAGCTTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605165950_57by TCCTCAGAAAATTGGACATAGTACTACCAGAG(+CAGTGT)GATCCCGCAATACTTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605165949_pr16 TTCTGGATATATGCCCAGGAGAAGTATTGCGG(+CAGTGT)GATCCTCTGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605165946_uf8t TCCTCAGAAAATTGGACATAGTACTACCGGAG(+CAGTGT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605165945_6h06 TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605165943_sykd GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10