Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165926_lx92 TGTGACCAGTTACATGGTCAGTTTTGGAAAAG(+CAGTGT)GTACCATGAGGTGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240605165925_wyoa AAGAAAATATGAGGCCAAGAATCTGGGGACAG(+CAGTGT)GATCCTTTTAGTCTCCATCTGAGCCTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605165924_9349 GTTAACTCTGAGGCTCAGATGGAGACTAAAAG(+CAGTGT)GATCCTGTCCCCAGATTCTTGGCCTCATATT NGG 10 8 17 8 24 0 100 5 10
    240605165921_62x8 GGATGGACCATGTAGAGACTGTCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605165920_o646 GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATATGACAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605165918_lzzp CGTGGGTGGGGTCGATGGTCAGCTACATTGAG(+CAGTGT)GATCCAAGAATGGCAGCAGTGCCTGCACCCC NGG 10 8 17 8 24 0 100 5 10
    240605165917_bbzw CCAGTGGGGTGCAGGCACTGCTGCCATTCTTG(+CAGTGT)GATCCTCAATGTAGCTGACCATCGACCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605165915_wvew GCATATAAGCCCCCCTGTTCTATGAGATCTGG(+CAGTGT)GTACCCTCACTGCCTTCCCAGCTCCGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605165914_6vvy CATGGAGACTCGGAGCTGGGAAGGCAGTGAGG(+CAGTGT)GTACCCAGATCTCATAGAACAGGGGGGCTTA NGG 10 8 17 8 24 0 100 5 10
    240605165911_z75a AAAATGTCCTTGATAATAAATGGTATGGCCCG(+CAGTGT)GATCCTATTTTGATAAGATCCAGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10