Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165330_flrs AATGCAACAGTATTCTGAGGTGGGGCCTTAGG(+CAGTGT)GATCCAACTGCATCATGTGGTTTGATCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605165328_tg4e CTGGGAGAATGAGGGCCCACTGAACAAGCAGG(+CAGTGT)GATCCAATTCACACATCAAAGTCTGTAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605165327_n1oy TCTTCTCTTTACAGACTTTGATGTGTGAATTG(+CAGTGT)GATCCCTGCTTGTTCAGTGGGCCCTCATTCT NGG 10 8 17 8 24 0 100 5 10
    240605165325_alkr AAGAATAAGAAACCAAGAGTCGAGAGATCCAG(+CAGTGT)GATCCCATTCTGCGGCTGCTGTTTGCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240605165323_gvd9 GGGTTACTCAGCAAACAGCAGCCGCAGAATGG(+CAGTGT)GATCCTGGATCTCTCGACTCTTGGTTTCTTA NGG 10 8 17 8 24 0 100 5 10
    240605165321_h9l1 CCTTGTGCTCTGTCAGGAGCCATAGCTCCTGG(+CAGTGT)GATCCTTAAGATAGAAGTGGGTGACCCGTGT NGG 10 8 17 8 24 0 100 5 10
    240605165320_1ow9 AGTGTACACGGGTCACCCACTTCTATCTTAAG(+CAGTGT)GATCCCAGGAGCTATGGCTCCTGACAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605165318_cw1m AATCTTCTCTACCCAAATCCTCCTCATAAATG(+CAGTGT)GATCCATGATGACAGCTAAGAGAAAAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605165317_kfgt TACTTATTTTTTTTCTCTTAGCTGTCATCATG(+CAGTGT)GATCCATTTATGAGGAGGATTTGGGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605165316_hmt5 TGCAAAGGACTGGAGATACAGGGTTAAGACAG(+CAGTGT)GTACCATCTACATATATTCTGAACAATGCAG NGG 10 8 17 8 24 0 100 5 10