Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165416_7zm0 TGTGACCAATTATATGGTTAATTTTGGAGATG(+CAGTGT)GTACCATGAGGCACTGAGAAGAAAGTATATT NGG 10 8 17 8 24 0 100 5 10
    240605165414_hmvn AGATTATTGCCACACATTGTAGGGAGGCAGAG(+CAGTGT)GTACCATGCCTTGCCCTGGGTATGCCTTGCC NGG 10 8 17 8 24 0 100 5 10
    240605165413_dtdj GTGGTGGCAAGGCATACCCAGGGCAAGGCATG(+CAGTGT)GTACCTCTGCCTCCCTACAATGTGTGGCAAT NGG 10 8 17 8 24 0 100 5 10
    240605165412_onyi AAGAAACAGTCTTTTTATAGCAAGCCAGACTG(+CAGTGT)GTACCTGGCTGTTCCTAGGTAACTGTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605165410_yz0c GCTCCACCCAACAGTTACCTAGGAACAGCCAG(+CAGTGT)GTACCAGTCTGGCTTGCTATAAAAAGACTGT NGG 10 8 17 8 24 0 100 5 10
    240605165409_iwni TAGATCGTTAACCTGATCCTGATTTAACTTTG(+CAGTGT)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605165407_nudr ACGAAATGGACAAATTTCTGGACAGATACCAG(+CAGTGT)GTACCAAAGTTAAATCAGGATCAGGTTAACG NGG 10 8 17 8 24 0 100 5 10
    240605165405_vqa5 CAAATGGTTTATTTTAAACTAGACCCATCATG(+CAGTGT)GTACCTCAGGGAAAGATGTCTCAGCATGGAC NGG 10 8 17 8 24 0 100 5 10
    240605165403_z8la AGTGGGTCCATGCTGAGACATCTTTCCCTGAG(+CAGTGT)GTACCATGATGGGTCTAGTTTAAAATAAACC NGG 10 8 17 8 24 0 100 5 10
    240605165402_o2jj TACCCAGGGAGATGCGAGGAGATAGATTCATG(+CAGTGT)GTACCTGAGCACAGGTAACCAGCCACATGGT NGG 10 8 17 8 24 0 100 5 10