Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165504_yiku CCAGAAATGCCATTCTTGATTAGGACTGGGTG(+CAGTGT)GTACCAGTGAGAGCCTTACAATGTGATGAGC NGG 10 8 17 8 24 0 100 5 10
    240605165502_p95l CCTCAGGCCTGATCCATCATGCCCTGGACGCG(+CAGTGT)GTACCCTAGAGCTTCTTAGCTGACTCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605165501_0bwd GTGACAGCCAGAGTCAGCTAAGAAGCTCTAGG(+CAGTGT)GTACCGCGTCCAGGGCATGATGGATCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605165459_rho3 CTCAACGCAAACGAAGGGACTAGCTTATCCAG(+CAGTGT)GATCCAACGTGCGACAGGGATAGCCTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605165457_zmcv AGTGCTTTTAAGGCTATCCCTGTCGCACGTTG(+CAGTGT)GATCCTGGATAAGCTAGTCCCTTCGTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240605165456_8e7v GAATGCAAAAATAACTGCATTTTCCTGTTCAG(+CAGTGT)GATCCAGTTGTATTTGACCATTCTAAATTAG NGG 10 8 17 8 24 0 100 5 10
    240605165455_deso TTTTACTAATTTAGAATGGTCAAATACAACTG(+CAGTGT)GATCCTGAACAGGAAAATGCAGTTATTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605165452_oiqe ATTCTTTCACCAAGGAAGCAATGGTGGCCAGG(+CAGTGT)GTACCAGTCAGAGCCATTATTGCTCAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605165451_kpos AGTAATTCTCTGAGCAATAATGGCTCTGACTG(+CAGTGT)GTACCCTGGCCACCATTGCTTCCTTGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605165450_z0p3 AGGAGTAATGTAAGGCTAGCTAAACTCTAGTG(+CAGTGT)GTACCAACGGAAGACTTCCGGTACGCCAGGC NGG 10 8 17 8 24 0 100 5 10