Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165448_emi0 ACCCCGCCTGGCGTACCGGAAGTCTTCCGTTG(+CAGTGT)GTACCACTAGAGTTTAGCTAGCCTTACATTA NGG 10 8 17 8 24 0 100 5 10
    240605165447_zpe1 GTCCTTGGTTGTGAAGTATCATCCCAGTCATG(+CAGTGT)GTACCCTGTGCTGGCAACAGAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605165445_unws TATTTTTTTTTTTTTTCTGTTGCCAGCACAGG(+CAGTGT)GTACCATGACTGGGATGATACTTCACAACCA NGG 10 8 17 8 24 0 100 5 10
    240605165443_joh8 AGGCCTCACATCCCCACTCTGCTCCCCGCCTG(+CAGTGT)GTACCTGTGTTAATTTAGACCTTCACCACCT NGG 10 8 17 8 24 0 100 5 10
    240605165442_dr1r GAGGCAGGTGGTGAAGGTCTAAATTAACACAG(+CAGTGT)GTACCAGGCGGGGAGCAGAGTGGGGATGTGA NGG 10 8 17 8 24 0 100 5 10
    240605165439_2rn3 ATAAGTCCCCATTTGAGGTCTAGGTTCCCAGG(+CAGTGT)GATCCATTTACCTGGCCAGCCAGAAGCCCCT NGG 10 8 17 8 24 0 100 5 10
    240605165437_d856 TATGCAGGGGCTTCTGGCTGGCCAGGTAAATG(+CAGTGT)GATCCCTGGGAACCTAGACCTCAAATGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605165435_wh1k CAATAAACTTTCTTGAATCTTCGAGTCTCCTG(+CAGTGT)GATCCCCATGGCCTCCATCACATCTTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605165433_w3ux ACATTAGAAGAAGATGTGATGGAGGCCATGGG(+CAGTGT)GATCCAGGAGACTCGAAGATTCAAGAAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605165431_jq0w TTAGATCGATTTTTTTTAAAAGTATGTGTTGG(+CAGTGT)GTACCTTTTCTGTGTGTATGCTCAGGCAGGC NGG 10 8 17 8 24 0 100 5 10