Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165400_9lki ATTCTACCATGTGGCTGGTTACCTGTGCTCAG(+CAGTGT)GTACCATGAATCTATCTCCTCGCATCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605165359_z3gf GGCTAACTGTAGCTGTATGTTAACAGTGGCAG(+CAGTGT)GTACCCAGCTCCTCTCCCTGAGGCTACAGCT NGG 10 8 17 8 24 0 100 5 10
    240605165357_2ch6 ATGGCAGCTGTAGCCTCAGGGAGAGGAGCTGG(+CAGTGT)GTACCTGCCACTGTTAACATACAGCTACAGT NGG 10 8 17 8 24 0 100 5 10
    240605165355_ttx8 TAGTAGGAGTGGGAAATGGATTACTTTGTCTG(+CAGTGT)GATCCCGAGTGGCTTTAGATTACACTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605165354_sgx4 ATGTCCCCTGAGTGTAATCTAAAGCCACTCGG(+CAGTGT)GATCCAGACAAAGTAATCCATTTCCCACTCC NGG 10 8 17 8 24 0 100 5 10
    240605165352_e7ei TGGCAGAGCCTCTCAGGAGACAGCTATATCGG(+CAGTGT)GATCCTGTTAGCAAACACTTCTTGGCATCCA NGG 10 8 17 8 24 0 100 5 10
    240605165351_ctz0 TATTGTGGATGCCAAGAAGTGTTTGCTAACAG(+CAGTGT)GATCCCGATATAGCTGTCTCCTGAGAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605165350_7j25 GGCAACTAGCTTACAGTGAAGAGGAGGCTGAG(+CAGTGT)GATCCAAGCTCAGGCACTCTAGGCTACAAAA NGG 10 8 17 8 24 0 100 5 10
    240605165348_lg49 ACCCCTTTTGTAGCCTAGAGTGCCTGAGCTTG(+CAGTGT)GATCCTCAGCCTCCTCTTCACTGTAAGCTAG NGG 10 8 17 8 24 0 100 5 10
    240605165346_305d CCCTGCCTTCACTGTAAAAGATCCCTAGGCAG(+CAGTGT)GATCCTCTCACTACATATCCCCAAACTATAG NGG 10 8 17 8 24 0 100 5 10