Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164828_5mri GGAATGACCATCCAGAGACTGCTCTACCTGGG(+CAGTGT)GATCCATTCCATTATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605164827_38m8 TTCTGGGTATGTGTCCAGGAGAATAATTGCTG(+CAGTGT)GATCCTCCTGTAGTACTGTGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605164826_uigj TACTCAGAAAATAGTTCACAGTACTACAGGAG(+CAGTGT)GATCCAGCAATTATTCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10
    240605164824_j7np CACAATTTTCCTTTTCATCCCCCACCACATTG(+CAGTGT)GATCCTAAATATATACCATCACTTTTCTCGT NGG 10 8 17 8 24 0 100 5 10
    240605164823_e06a CCTCTACGAGAAAAGTGATGGTATATATTTAG(+CAGTGT)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605164822_ey7j ACTGTCTGGGTTTGGTGACTGATGATGGAATG(+CAGTGT)GATCCCCAGGTAGAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605164819_0hnm GGAATGACCATCCAGAGACTGCTCTACCTGGG(+CAGTGT)GATCCATTCCATCATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605164818_2kv5 TTCTGGGTATGTGTCCAGGAGAATAATTGCTG(+CAGTGT)GATCCTCCTGTAGTACTATGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605164816_z2xx TACTCAGAAAATAGTTCATAGTACTACAGGAG(+CAGTGT)GATCCAGCAATTATTCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10
    240605164815_8ojt CACAATTTTCCTTTTCATCCCCCACCACATTG(+CAGTGT)GATCCTAAATATATACCATCACTTTTCTCAT NGG 10 8 17 8 24 0 100 5 10