Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164813_oif2 CCTCTATGAGAAAAGTGATGGTATATATTTAG(+CAGTGT)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605164812_7fmo GTAAAAGGTCTACAATACCAGATGCGAGTCGG(+CAGTGT)GATCCAAATCACCCAGCCTAACTGTGCTTAG NGG 10 8 17 8 24 0 100 5 10
    240605164810_78tv TGTAGCTAAGCACAGTTAGGCTGGGTGATTTG(+CAGTGT)GATCCCGACTCGCATCTGGTATTGTAGACCT NGG 10 8 17 8 24 0 100 5 10
    240605164809_vs65 ATGGAGAGGGTGTGCACTAACTGAAACTTTTG(+CAGTGT)GATCCAAGGACAAGACAATCTTTTCCTTATC NGG 10 8 17 8 24 0 100 5 10
    240605164807_3kmp AAACTGATAAGGAAAAGATTGTCTTGTCCTTG(+CAGTGT)GATCCAAAAGTTTCAGTTAGTGCACACCCTC NGG 10 8 17 8 24 0 100 5 10
    240605164806_mdw3 GTGCTAAAGAGATTTTCACAGACATCTTCCAG(+CAGTGT)GTACCTGGTCTAGTTAGTGGCAGGGATATGT NGG 10 8 17 8 24 0 100 5 10
    240605164804_be8s GTAAAACATATCCCTGCCACTAACTAGACCAG(+CAGTGT)GTACCTGGAAGATGTCTGTGAAAATCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605164802_9wbv TCGGTGTGGAAAAAAGATGACCAGAAAGTCAG(+CAGTGT)GTACCCCGAATCACTGGAAACTCCTTTCTTA NGG 10 8 17 8 24 0 100 5 10
    240605164800_banb CTACATAAGAAAGGAGTTTCCAGTGATTCGGG(+CAGTGT)GTACCTGACTTTCTGGTCATCTTTTTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240605164759_f4gb TTTTATTAATGTTGAATATTTTCTATCTAGTG(+CAGTGT)GATCCTTGAGAAAGTAATGTCCTACTTGAAT NGG 10 8 17 8 24 0 100 5 10