Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164713_rvyx GGGCTAGGTTTAGCCACTAGGGTATCCTGCAG(+CAGTGT)GATCCAAAGGGGCTGTAGGGCCAATTCTGAC NGG 10 8 17 8 24 0 100 5 10
    240605164711_gdr0 TGGGGCAGCTGTCAAAAGCTGCAGTTGGATAG(+CAGTGT)GTACCAGCCTGTGGTGACTGGTAAGTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605164710_5ovm GTTTGCCACTACTTACCAGTCACCACAGGCTG(+CAGTGT)GTACCTATCCAACTGCAGCTTTTGACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605164708_5if2 GTCCCAGTTTCCACCTTCCTCTGGCCCAGTAG(+CAGTGT)GATCCCCAATCTGACTCTTTCAAGGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605164707_s4x7 GAATGTCTGAGCCTTGAAAGAGTCAGATTGGG(+CAGTGT)GATCCTACTGGGCCAGAGGAAGGTGGAAACT NGG 10 8 17 8 24 0 100 5 10
    240605164705_3upm GGTAACCACATTTTTCAACTCCACCCACCATG(+CAGTGT)GTACCCTAGAACCAAGACTGGAATACTGACT NGG 10 8 17 8 24 0 100 5 10
    240605164703_t8d5 TCTCCAGTCAGTATTCCAGTCTTGGTTCTAGG(+CAGTGT)GTACCATGGTGGGTGGAGTTGAAAAATGTGG NGG 10 8 17 8 24 0 100 5 10
    240605164701_4zz6 ACCACATGGTGGCCCACAACCATCTGTAATGG(+CAGTGT)GATCCAATGCCCTCTTTTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605164700_1fmc GCTGTCTTCAGACACACCAAAAGAGGGCATTG(+CAGTGT)GATCCCATTACAGATGGTTGTGGGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605164659_limt TTAGAGTGGTGGGTTGGTCAGCTGGAAGACAG(+CAGTGT)GATCCAAAATGTTCCATGGAAGGCTGTCTTT NGG 10 8 17 8 24 0 100 5 10