Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164623_a16k GTATGAACTCACACATCTCTTAATGGAGTATG(+CAGTGT)GATCCATTGTAAAATCTCAGCATTTCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605164621_5uqx ACCCCCTGCAGTGGGCTAATTAGGACTGAGGG(+CAGTGT)GTACCTCTAAGGCTTTGGGGGTTCAACAGAG NGG 10 8 17 8 24 0 100 5 10
    240605164620_dsw4 GAGGCCTCTGTTGAACCCCCAAAGCCTTAGAG(+CAGTGT)GTACCCCTCAGTCCTAATTAGCCCACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605164618_mu8o AGAAGGGGACCCTGGCCACAGGACACTTCCAG(+CAGTGT)GATCCATGCTCCCCTCCTCCTTGGGTCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605164617_gj06 TCAAACTCAGACCCAAGGAGGAGGGGAGCATG(+CAGTGT)GATCCTGGAAGTGTCCTGTGGCCAGGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240605164615_rjp8 GCTCCTGAGACTTCCCACTTTTAGCAGTTGGG(+CAGTGT)GTACCAGGCCAGCAGCACCAGGGGCCAGATG NGG 10 8 17 8 24 0 100 5 10
    240605164613_rwv7 CAACCCATCTGGCCCCTGGTGCTGCTGGCCTG(+CAGTGT)GTACCCCAACTGCTAAAAGTGGGAAGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605164612_10d8 TGAGCCATGCCTCCAGGTAACTGATGGATTTG(+CAGTGT)GTACCTCTCATCCCAGGCTTTTTAAGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605164610_5j0m CCAAGGCTTTCTTAAAAAGCCTGGGATGAGAG(+CAGTGT)GTACCAAATCCATCAGTTACCTGGAGGCATG NGG 10 8 17 8 24 0 100 5 10
    240605164608_ve8s CTGACATCCAATGGGTCACACCCACAAGTTGG(+CAGTGT)GTACCCGTGAGTTCACTCAGGTGTGTCTTTG NGG 10 8 17 8 24 0 100 5 10