Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164553_uc48 AATAGTTTTATTTCACACCTTGTTTCTAGCTG(+CAGTGT)GTACCACTTGCATGATGCAACCAAAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240605164551_05c2 ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+CAGTGT)GTACCTTGTGCTGTGGCTGGACTTGGTAGTG NGG 10 8 17 8 24 0 100 5 10
    240605164549_pu47 TTACACACTACCAAGTCCAGCCACAGCACAAG(+CAGTGT)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10
    240605164548_wfm5 TCTTTTGGAGTTGTCCAAAAGATCGTGAGTTG(+CAGTGT)GATCCCAGACATTGGACAGTTGGAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605164547_g14f TAAAAATTAAACTCCAACTGTCCAATGTCTGG(+CAGTGT)GATCCAACTCACGATCTTTTGGACAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240605164545_zypg TTACAAACTACCAAGTCCAGCCACAGCACAAG(+CAGTGT)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10
    240605164544_rvn8 ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+CAGTGT)GTACCTTGTGCTGTGGCTGGACTTGGTAGTT NGG 10 8 17 8 24 0 100 5 10
    240605164542_k9fe GAAAAATTAAATTCCAACTGTCCAACGTCTGG(+CAGTGT)GATCCAATTCATGATCTTCTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605164541_0dme GCCCTTGGAGGAGCCCAGAAGATCATGAATTG(+CAGTGT)GATCCCAGACGTTGGACAGTTGGAATTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605164540_m77d AACAAAAGGATATACCTTCTCAGCACCTCATG(+CAGTGT)GTACCTTCTCCAAAATCGACCAAGAGAACCA NGG 10 8 17 8 24 0 100 5 10