Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164639_0qcf CCACCATCCAGACAACAGCCTGTCACCTTCGG(+CAGTGT)GATCCAGGGGAAGGGAAGAAAGGTCCCTCTA NGG 10 8 17 8 24 0 100 5 10
    240605164637_uu2m TGGGAGTACAGAAGGAGCTGAAGGTATAGAGG(+CAGTGT)GATCCCAGACTGTGGCTGGACCTGGCTATGC NGG 10 8 17 8 24 0 100 5 10
    240605164636_n51p TAGCTGCATAGCCAGGTCCAGCCACAGTCTGG(+CAGTGT)GATCCCTCTATACCTTCAGCTCCTTCTGTAC NGG 10 8 17 8 24 0 100 5 10
    240605164634_xp76 CAGGTCTTCCCTAGAGTCCTAAGGGCATCGAG(+CAGTGT)GATCCCATCTGCCATGTTTCAGTCTGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605164633_lkre CAGTGGAGGGCAGACTGAAACATGGCAGATGG(+CAGTGT)GATCCTCGATGCCCTTAGGACTCTAGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605164631_csbx TGTATGATCTGGGACAGGCTGAAGCAGGCAGG(+CAGTGT)GTACCCACTCTGCAGTGTAGTTCCTGGAGCG NGG 10 8 17 8 24 0 100 5 10
    240605164629_wueb CTTCTCGCTCCAGGAACTACACTGCAGAGTGG(+CAGTGT)GTACCCTGCCTGCTTCAGCCTGTCCCAGATC NGG 10 8 17 8 24 0 100 5 10
    240605164628_atoa GTTACACCTAGGCTGCAGGAAGGATGCTGGAG(+CAGTGT)GTACCCTGGCCTCACCTGGTTTAGGACAGGA NGG 10 8 17 8 24 0 100 5 10
    240605164626_6hws CACATTCCTGTCCTAAACCAGGTGAGGCCAGG(+CAGTGT)GTACCTCCAGCATCCTTCCTGCAGCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240605164624_o3a1 ACGGCCCCAAGAAATGCTGAGATTTTACAATG(+CAGTGT)GATCCATACTCCATTAAGAGATGTGTGAGTT NGG 10 8 17 8 24 0 100 5 10