Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605164726_5k50 GTTCAGTCGACCCTGAACTTTAACTTACACAG(+CAGTGT)GTACCAATAGAGTGTGTTGCCGTGTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605164725_ojqd GATGCACCAGGTGAACCTTGGGCATCTAACTG(+CAGTGT)GATCCTCATTTATTACCAAAGATAGTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605164724_8k2l TATGAAACCAACTATCTTTGGTAATAAATGAG(+CAGTGT)GATCCAGTTAGATGCCCAAGGTTCACCTGGT NGG 10 8 17 8 24 0 100 5 10
    240605164722_v49a TTTTTTATTTTTAAATTTTTTTTTAAGCACGG(+CAGTGT)GATCCTGTCTTCACTCCTACACACTGAACAT NGG 10 8 17 8 24 0 100 5 10
    240605164721_7zhr TGGATATGTTCAGTGTGTAGGAGTGAAGACAG(+CAGTGT)GATCCCGTGCTTAAAAAAAAATTTAAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605164720_1o3u GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+CAGTGT)GATCCCATTACAGATGGTTGTGAGCAATTGT NGG 10 8 17 8 24 0 100 5 10
    240605164718_34od GCCACACAATTGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605164717_5zf2 ACCTTTACAGACTTGCCCCTTGCCTCACACAG(+CAGTGT)GATCCAATCAAATTTAATTCAAGTCTCAATG NGG 10 8 17 8 24 0 100 5 10
    240605164716_1t2s TTTCACATTGAGACTTGAATTAAATTTGATTG(+CAGTGT)GATCCTGTGTGAGGCAAGGGGCAAGTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605164714_f50a AATGAGTCAGAATTGGCCCTACAGCCCCTTTG(+CAGTGT)GATCCTGCAGGATACCCTAGTGGCTAAACCT NGG 10 8 17 8 24 0 100 5 10