Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162343_42y4 ACCCCAGGAACTCCTAAACAGTTTAGGAGCTG(+CAGTGT)GATCCTCCCACTCTGGGATGACCCAAATGTT NGG 10 8 17 8 24 0 100 5 10
    240605162341_es4t TCGGGAACATTTGGGTCATCCCAGAGTGGGAG(+CAGTGT)GATCCAGCTCCTAAACTGTTTAGGAGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605162339_rsmb CATCAGGACAAGATTTGTATAGGAATGCTGGG(+CAGTGT)GATCCAAACTCAAGTGTACTTATACCAACCA NGG 10 8 17 8 24 0 100 5 10
    240605162338_n8l0 GGACTTGGTTGGTATAAGTACACTTGAGTTTG(+CAGTGT)GATCCCCAGCATTCCTATACAAATCTTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605162337_onhx CAAACAGTTTAATAGCAAATAAACAAAGGAAG(+CAGTGT)GTACCACACTTGGCAGATACAAAATGACTTT NGG 10 8 17 8 24 0 100 5 10
    240605162336_x04l GACAAAAAGTCATTTTGTATCTGCCAAGTGTG(+CAGTGT)GTACCTTCCTTTGTTTATTTGCTATTAAACT NGG 10 8 17 8 24 0 100 5 10
    240605162334_2ka8 TGTACTCCCTGAGCAGAGCAGCCAGCCTCCTG(+CAGTGT)GATCCCCCACTGAGACCTGGAGGCCACCACA NGG 10 8 17 8 24 0 100 5 10
    240605162331_kmu4 CTCTTTGTGGTGGCCTCCAGGTCTCAGTGGGG(+CAGTGT)GATCCAGGAGGCTGGCTGCTCTGCTCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605162330_xwfs TCCCTGCATGTATGCCTGCAGGTCAGAAGAGG(+CAGTGT)GTACCAGATCCCGTTACAGATGGTTGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605162328_ww8i TGGTGGCTCACAACCATCTGTAACGGGATCTG(+CAGTGT)GTACCCTCTTCTGACCTGCAGGCATACATGC NGG 10 8 17 8 24 0 100 5 10