Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162257_2hm6 CTTTCAATCTCAATCACTCAACAGTAAATTTG(+CAGTGT)GTACCTTCTGTAGGCTCTGCCTTCTGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605162256_wr5o GGACTCTGCTCAGAAGGCAGAGCCTACAGAAG(+CAGTGT)GTACCAAATTTACTGTTGAGTGATTGAGATT NGG 10 8 17 8 24 0 100 5 10
    240605162254_zun4 AAAATCTGAATATTTTTTTAAGATCACCTAAG(+CAGTGT)GTACCATTTTAAAGATTCCAAGACCCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605162253_z7pz GAATTTTCTAGGGTCTTGGAATCTTTAAAATG(+CAGTGT)GTACCTTAGGTGATCTTAAAAAAATATTCAG NGG 10 8 17 8 24 0 100 5 10
    240605162251_y63j TGCCAGATGGGGAAAACAAGAAGGACAAATAG(+CAGTGT)GATCCTGCATTGTTTTTGCTTCCTCTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605162250_x9lf AATTGACAGGAGAGGAAGCAAAAACAATGCAG(+CAGTGT)GATCCTATTTGTCCTTCTTGTTTTCCCCATC NGG 10 8 17 8 24 0 100 5 10
    240605162248_jrbf CTACTCATAACTATCCTTATGACCTGAGTTGG(+CAGTGT)GTACCACATAGTTTTGTCACTGAAAAATGGA NGG 10 8 17 8 24 0 100 5 10
    240605162247_oa4p TGTAATCCATTTTTCAGTGACAAAACTATGTG(+CAGTGT)GTACCCAACTCAGGTCATAAGGATAGTTATG NGG 10 8 17 8 24 0 100 5 10
    240605162246_6aeb AAATGAAGCTGATAAAACAAAGCTAAGACTGG(+CAGTGT)GATCCTTGCAAGTATCTGATAAAACTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605162244_bvvk GAGTAAGGCTAGTTTTATCAGATACTTGCAAG(+CAGTGT)GATCCCAGTCTTAGCTTTGTTTTATCAGCTT NGG 10 8 17 8 24 0 100 5 10