Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162214_5vtx TGAAGACAGCCCATTTCACAGACAATGACATG(+CAGTGT)GTACCCAGCAGCATCTCCCCTTCTGCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605162212_ik27 GTCCTTACAAGCAGAAGGGGAGATGCTGCTGG(+CAGTGT)GTACCATGTCATTGTCTGTGAAATGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605162210_7szo AGGGATGTGTTCTATGGCCTGTGTGCTTTGTG(+CAGTGT)GTACCACCCAGCCCAGGCAGAAACTGGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605162209_mf5w TAACAAAGCCCAGTTTCTGCCTGGGCTGGGTG(+CAGTGT)GTACCACAAAGCACACAGGCCATAGAACACA NGG 10 8 17 8 24 0 100 5 10
    240605162207_rw1i TTTTTTTAAAAAAGAAACATGATTATGCATAG(+CAGTGT)GATCCATTTACATAATGCAAATAAGCTTTCG NGG 10 8 17 8 24 0 100 5 10
    240605162206_49tp GAGAACGAAAGCTTATTTGCATTATGTAAATG(+CAGTGT)GATCCTATGCATAATCATGTTTCTTTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605162205_4c79 TTTAAGGAATATGGTTGCAGCAAAACAGAAAG(+CAGTGT)GATCCAAAAGTGGTTAGGAGCCTTGTCTAAT NGG 10 8 17 8 24 0 100 5 10
    240605162204_j5rc AGTGCATTAGACAAGGCTCCTAACCACTTTTG(+CAGTGT)GATCCTTTCTGTTTTGCTGCAACCATATTCC NGG 10 8 17 8 24 0 100 5 10
    240605162203_3h5q AACAAGAAAATTTGCTTGCTAACTCTTCACTG(+CAGTGT)GTACCAGAATATTTTTAGAGCTAAAATGCCA NGG 10 8 17 8 24 0 100 5 10
    240605162201_2985 CAGCTTGGCATTTTAGCTCTAAAAATATTCTG(+CAGTGT)GTACCAGTGAAGAGTTAGCAAGCAAATTTTC NGG 10 8 17 8 24 0 100 5 10