Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162129_df8l ACATGTGGTTGCCATGGCTTAATGTATGGACG(+CAGTGT)GATCCTCCACTGTCTTGCAATTCATTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605162128_q2h1 TTTTTCAACAAATGAATTGCAAGACAGTGGAG(+CAGTGT)GATCCGTCCATACATTAAGCCATGGCAACCA NGG 10 8 17 8 24 0 100 5 10
    240605162126_o09t CACCTTTTCTACAGCAATTTTTAGTAATTATG(+CAGTGT)GTACCAAGTTCCCACAAACCTTTGGGTGACT NGG 10 8 17 8 24 0 100 5 10
    240605162125_0zr2 TACCCAGTCACCCAAAGGTTTGTGGGAACTTG(+CAGTGT)GTACCATAATTACTAAAAATTGCTGTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605162123_906w AGCCTCACCTGGTCCCTGGCCTTCAGCCTCTG(+CAGTGT)GATCCCTGGGTGGGACCCAGAGAATCTCACG NGG 10 8 17 8 24 0 100 5 10
    240605162120_i3ka ATGAGCGTGAGATTCTCTGGGTCCCACCCAGG(+CAGTGT)GATCCAGAGGCTGAAGGCCAGGGACCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605162119_lktt GTTCTTCAGTAGTCTCACACTGGATTGGTCTG(+CAGTGT)GATCCACACGGGTACTCACTTCAGGTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605162118_cvna TGTATTCAGGACCTGAAGTGAGTACCCGTGTG(+CAGTGT)GATCCAGACCAATCCAGTGTGAGACTACTGA NGG 10 8 17 8 24 0 100 5 10
    240605162116_m5qg ACGGAGCAGCCGGAAACAAGGAGGCCTCGTGG(+CAGTGT)GATCCTTTAGTGCCAAGCCTGGCGTCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605162114_j9pz CAAGTACAGACCGAGGCCCAGGAGAACGGGCG(+CAGTGT)GTACCGTGTCCAGTCAGCTGCATGGTACTGT NGG 10 8 17 8 24 0 100 5 10