Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162200_tqtx CTTTCCATCCTGAACCCATACTGCTGGATTTG(+CAGTGT)GATCCATGGACCTGAGGGCCATCAGCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605162158_j30l CAGGAGTGGGGCTGATGGCCCTCAGGTCCATG(+CAGTGT)GATCCAAATCCAGCAGTATGGGTTCAGGATG NGG 10 8 17 8 24 0 100 5 10
    240605162156_l1np CAATTTTAATGTTTCCTAAGCATTTTGACCAG(+CAGTGT)GTACCCAGGTTTAAGCTATGAACATTGACAG NGG 10 8 17 8 24 0 100 5 10
    240605162154_ayxj GGACACTGTCAATGTTCATAGCTTAAACCTGG(+CAGTGT)GTACCTGGTCAAAATGCTTAGGAAACATTAA NGG 10 8 17 8 24 0 100 5 10
    240605162152_gheu CCTTGAGACCTTTATAAGCAAAAGCAAACCAG(+CAGTGT)GTACCCACCAGTTTGCTCAGACCCAGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605162150_5e14 ACAAAGTCTGCTGGGTCTGAGCAAACTGGTGG(+CAGTGT)GTACCTGGTTTGCTTTTGCTTATAAAGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605162148_21zm AAAGTTAAAAATTCCTTAATTTTTTATTCCTG(+CAGTGT)GTACCACTACCACAATTTACAGGGCAATATA NGG 10 8 17 8 24 0 100 5 10
    240605162147_hpoj TCAGGTATATTGCCCTGTAAATTGTGGTAGTG(+CAGTGT)GTACCAGGAATAAAAAATTAAGGAATTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605162146_nf79 CACTCAGGCCTGGAACTCGGTGCCCCCTCTCG(+CAGTGT)GATCCTGGCCTCACATGGGCCTCAGACCTAA NGG 10 8 17 8 24 0 100 5 10
    240605162144_3f5z TTTTCTTAGGTCTGAGGCCCATGTGAGGCCAG(+CAGTGT)GATCCGAGAGGGGGCACCGAGTTCCAGGCCT NGG 10 8 17 8 24 0 100 5 10