Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162059_em8s CAGGTCAGTATTTTTCCATTTGTATCTCTCAG(+CAGTGT)GATCCAGCCGGAAACACTAGGTTCACTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605162058_5u8a AGGATACTTAAGTCATCAAAGACATTGGTATG(+CAGTGT)GATCCGCTGTGGTGGTACATGCTGTAATCTC NGG 10 8 17 8 24 0 100 5 10
    240605162056_o05z GTGCTGAGATTACAGCATGTACCACCACAGCG(+CAGTGT)GATCCATACCAATGTCTTTGATGACTTAAGT NGG 10 8 17 8 24 0 100 5 10
    240605162055_o6ep AGCACTGGGCTCGCTTGGCATGTCCTGGGTTG(+CAGTGT)GATCCCAGTACTCCAACTGAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605162054_kp1x CTTTTTTTTTTTTTTTTCAGTTGGAGTACTGG(+CAGTGT)GATCCAACCCAGGACATGCCAAGCGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605162052_hvco GCAAGCTGCTTCCCCCGAGCCTTCAGCACAGG(+CAGTGT)GATCCTCTCAACCACAGGCAGCCGGGTTGCC NGG 10 8 17 8 24 0 100 5 10
    240605162051_l62b TTGCTGGCAACCCGGCTGCCTGTGGTTGAGAG(+CAGTGT)GATCCCTGTGCTGAAGGCTCGGGGGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605162049_zxsi AAGGCATAGCAGTGGTACAGGGAACCCAGTGG(+CAGTGT)GTACCCCGCCATCTCTCTTCCAAAGATGAAC NGG 10 8 17 8 24 0 100 5 10
    240605162047_jlwi GGGAGGTTCATCTTTGGAAGAGAGATGGCGGG(+CAGTGT)GTACCCACTGGGTTCCCTGTACCACTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240605162046_w4oj TGTGTCAGCTACTGACAGAGCTGTGCTCTAGG(+CAGTGT)GTACCAGAGGGGCACAAGCATGGCCGATGAT NGG 10 8 17 8 24 0 100 5 10