Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162029_ipi7 CCTCCTTCCTGGTTGCATCCAAACTCCCCAGG(+CAGTGT)GTACCAAAGTGTGTGTAGAAAGGACTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605162027_1hfh CAAGACCTTCAAGAAGGTGGGCTCCGGGGATG(+CAGTGT)GTACCATTGCTCTTTCCTAGCTAACCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605162026_7lym CCGTATCTAGGGTTAGCTAGGAAAGAGCAATG(+CAGTGT)GTACCATCCCCGGAGCCCACCTTCTTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605162024_ls9m AAATGGTCCATATAAATTTGTGGCATCAATGG(+CAGTGT)GATCCCAACTTGGCTTCCCTAGTCTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605162023_nk0s CGTGGGAAGGAAGACTAGGGAAGCCAAGTTGG(+CAGTGT)GATCCCATTGATGCCACAAATTTATATGGAC NGG 10 8 17 8 24 0 100 5 10
    240605162021_2a9p AGGACCATGACAAAGCCCCTGAGATCAAGGTG(+CAGTGT)GTACCAGTTGCAGCAGCAATGTGGTGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605162020_zrvn TAAGGCTGAGCACCACATTGCTGCTGCAACTG(+CAGTGT)GTACCACCTTGATCTCAGGGGCTTTGTCATG NGG 10 8 17 8 24 0 100 5 10
    240605162018_cuf0 TGATGTGACTAGAAAGGCCTGGCCACTTTAGG(+CAGTGT)GTACCAAGGGAGAACTCCAAGGAGCATTTTT NGG 10 8 17 8 24 0 100 5 10
    240605162015_3kxd GGGGGAAAAATGCTCCTTGGAGTTCTCCCTTG(+CAGTGT)GTACCCTAAAGTGGCCAGGCCTTTCTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605162013_wwmz ATTGCCTTATGTAGTAATCAGCAACTGAAGGG(+CAGTGT)GTACCCAACCTCTACAGTCTTCCAGCACAAT NGG 10 8 17 8 24 0 100 5 10