Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161941_9ejh ATCCATCATAAATCATAAATGAATTCAGTGAG(+CAGTGT)GTACCAAGATACAAAATCATTATGCAAAACT NGG 10 8 17 8 24 0 100 5 10
    240605161940_jk06 TATAATAAAATAATACTAAAAGAGAGCATGTG(+CAGTGT)GATCCATACACCAGATTAATGCGGGGATAGG NGG 10 8 17 8 24 0 100 5 10
    240605161939_mer1 CATACCCTATCCCCGCATTAATCTGGTGTATG(+CAGTGT)GATCCACATGCTCTCTTTTAGTATTATTTTA NGG 10 8 17 8 24 0 100 5 10
    240605161937_mnqs CGACAAACGTGCATTTGATAGCACTATTTTAG(+CAGTGT)GTACCCAGAAGCAGACAATACAGCAGTGAAT NGG 10 8 17 8 24 0 100 5 10
    240605161936_kzyi GCCATATTCACTGCTGTATTGTCTGCTTCTGG(+CAGTGT)GTACCTAAAATAGTGCTATCAAATGCACGTT NGG 10 8 17 8 24 0 100 5 10
    240605161935_w8kk CGTCTACTTAAGCTATGTCCAGAGGCGTGCTG(+CAGTGT)GATCCTGCAGAGAAACAGTATGGCTTTATGA NGG 10 8 17 8 24 0 100 5 10
    240605161933_zq1t GAGTTTCATAAAGCCATACTGTTTCTCTGCAG(+CAGTGT)GATCCAGCACGCCTCTGGACATAGCTTAAGT NGG 10 8 17 8 24 0 100 5 10
    240605161932_psx0 GGGGGGGCACGGAGGAAAGCAAGGCTGTGTGG(+CAGTGT)GATCCACTGGAGCCTCAGGAGGAAGGGAACA NGG 10 8 17 8 24 0 100 5 10
    240605161930_aduy CCCAGTGTTCCCTTCCTCCTGAGGCTCCAGTG(+CAGTGT)GATCCCACACAGCCTTGCTTTCCTCCGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605161928_pnnt GTATTGGTGCAGAGGAGAGCTCTTGGTGCTGG(+CAGTGT)GATCCTATGCCACTCCCCACTCCATCTCTAA NGG 10 8 17 8 24 0 100 5 10