Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161855_r199 GGGTAAAAGTTCAGGTTTCACCTGCAGCCCTG(+CAGTGT)GTACCCTAGGCTAGACCACCTTCCCGGTATA NGG 10 8 17 8 24 0 100 5 10
    240605161854_kzso CCTGGCCTGTTGTCCTTTACCTACCTAAGCTG(+CAGTGT)GTACCTTTATTACTGTTGCATTGTACTGAAC NGG 10 8 17 8 24 0 100 5 10
    240605161853_h4wg ACTGGGTTCAGTACAATGCAACAGTAATAAAG(+CAGTGT)GTACCAGCTTAGGTAGGTAAAGGACAACAGG NGG 10 8 17 8 24 0 100 5 10
    240605161851_db0s GTAACCCCCTCCTTCCAACTTCTTAGGAGAAG(+CAGTGT)GATCCAAGCACGTATCTGTGCACACAAATTC NGG 10 8 17 8 24 0 100 5 10
    240605161850_tknk GTGTAGAATTTGTGTGCACAGATACGTGCTTG(+CAGTGT)GATCCTTCTCCTAAGAAGTTGGAAGGAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605161849_4trb TGGTTCAAGAGGTGGGCTGCACCTGTCTGGAG(+CAGTGT)GATCCCTGCATGCCATCAATAAGACTCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605161847_lcw7 TGTGACACTGAGTCTTATTGATGGCATGCAGG(+CAGTGT)GATCCTCCAGACAGGTGCAGCCCACCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605161845_0rvk AATTCATAGTTATTTATTTTATTCACATTAGG(+CAGTGT)GATCCTGAGGAAGTAGAATCTATATAGGACA NGG 10 8 17 8 24 0 100 5 10
    240605161843_xz1m ATACCTGTCCTATATAGATTCTACTTCCTCAG(+CAGTGT)GATCCCTAATGTGAATAAAATAAATAACTAT NGG 10 8 17 8 24 0 100 5 10
    240605161842_lpu9 ACCCCAGAGGTAGTATGGCAAAAACGAGCATG(+CAGTGT)GATCCTATCAGGTCACAACCGACAAAGTAAT NGG 10 8 17 8 24 0 100 5 10