Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161913_ipo5 GAATGACCGATTAGTAGCTGAATTGGCATAAG(+CAGTGT)GATCCACAGAAGGAAAGTCTGACCATTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605161911_zgef AATATGAGGGAGGCACATTTTCTAGTGGCTGG(+CAGTGT)GATCCTCTTCCAAACCATAAGAAAAAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605161910_gq3g ACCACTGCCTTTTTTCTTATGGTTTGGAAGAG(+CAGTGT)GATCCCAGCCACTAGAAAATGTGCCTCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605161908_bzxu ACAGGGTTAAAGTTTTGGTCCTCAGCCCATTG(+CAGTGT)GTACCATTAGGAAACAGGGTCTAGTCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605161907_22nh GAAATTCTCTGACTAGACCCTGTTTCCTAATG(+CAGTGT)GTACCAATGGGCTGAGGACCAAAACTTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605161906_6k78 CGGACTAGGTGGTTAGTGGATACTATTGTTTG(+CAGTGT)GATCCTGACTGTTACAGGGTTAAAGTTTTGG NGG 10 8 17 8 24 0 100 5 10
    240605161904_arrp GAGGACCAAAACTTTAACCCTGTAACAGTCAG(+CAGTGT)GATCCAAACAATAGTATCCACTAACCACCTA NGG 10 8 17 8 24 0 100 5 10
    240605161901_qydf GAGAAAGCCTAGAGTCTCCCCTTACTCCAGGG(+CAGTGT)GTACCAGCTTCAGAAGAAGGTGTGAGCTCAC NGG 10 8 17 8 24 0 100 5 10
    240605161900_78da GAAGTGTGAGCTCACACCTTCTTCTGAAGCTG(+CAGTGT)GTACCCCTGGAGTAAGGGGAGACTCTAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605161857_9ztj CCTGGTATACCGGGAAGGTGGTCTAGCCTAGG(+CAGTGT)GTACCAGGGCTGCAGGTGAAACCTGAACTTT NGG 10 8 17 8 24 0 100 5 10