Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161827_bcdh CTTACGAAGAAAGTGGAATTGATGGCATGTAG(+CAGTGT)GTACCAGAGTGCTTTAAAAAACATTATTACA NGG 10 8 17 8 24 0 100 5 10
    240605161825_voal ATATAATTTTAGTTTAGCAATAATTTTGACTG(+CAGTGT)GTACCAATAACTGTTTTTTAAAATTCCATAC NGG 10 8 17 8 24 0 100 5 10
    240605161824_1z7t CATAAGTATGGAATTTTAAAAAACAGTTATTG(+CAGTGT)GTACCAGTCAAAATTATTGCTAAACTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605161822_zh3h TAATAACAGACCAGCGATTTTATAGAGGCCAG(+CAGTGT)GATCCTGAACTGGGGCATCCTCTCTGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605161821_879y GCCTGCACTCCAGAGAGGATGCCCCAGTTCAG(+CAGTGT)GATCCTGGCCTCTATAAAATCGCTGGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605161820_rtn2 TTGGACTTTGTAAGCCTGAAAAAGAATATTTG(+CAGTGT)GATCCCTCTACTAAACAAGGGCTATGTATTA NGG 10 8 17 8 24 0 100 5 10
    240605161818_l14q CTGTATAATACATAGCCCTTGTTTAGTAGAGG(+CAGTGT)GATCCAAATATTCTTTTTCAGGCTTACAAAG NGG 10 8 17 8 24 0 100 5 10
    240605161817_67h9 TAAGGATAAATGCATTAAGTATAACTAATTTG(+CAGTGT)GTACCATACAAGTATTTTCTTTGGAATTTTG NGG 10 8 17 8 24 0 100 5 10
    240605161816_jxk0 ACATACAAAATTCCAAAGAAAATACTTGTATG(+CAGTGT)GTACCAAATTAGTTATACTTAATGCATTTAT NGG 10 8 17 8 24 0 100 5 10
    240605161815_95h4 ATGCCCCTTTGTAATTTTTCTTGCCTTCTGTG(+CAGTGT)GTACCTGCTCTCTAAAGCAGTAGTTTCTCCA NGG 10 8 17 8 24 0 100 5 10