Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161746_f7fy TAACTCTACTTTCACTAGATGTATCATTGTAG(+CAGTGT)GATCCTGCTAGTTTAATAACTGAGTTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605161744_awvu ATTAGTTCTGATTCATCCCCGAGGGAAGAGAG(+CAGTGT)GTACCCATGGCTTCAGTGAGCACATGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605161742_lcpk CTGGGCTTGGCATGTGCTCACTGAAGCCATGG(+CAGTGT)GTACCTCTCTTCCCTCGGGGATGAATCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605161741_0npg TGGCAAGTGGCCAGCTGAGCCCAGGTTACTTG(+CAGTGT)GTACCCACTCTGCCTTGAGCCTTCCTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605161739_6v5s CCCTGGGGGAAGGAAGGCTCAAGGCAGAGTGG(+CAGTGT)GTACCAAGTAACCTGGGCTCAGCTGGCCACT NGG 10 8 17 8 24 0 100 5 10
    240605161737_tfp5 CCGCTGAGATCCAGCATCCAGCTGTGGCCAGG(+CAGTGT)GATCCACCACCTCTGTGTACACCCTCACCCG NGG 10 8 17 8 24 0 100 5 10
    240605161735_9cqj AGCAGCGGGTGAGGGTGTACACAGAGGTGGTG(+CAGTGT)GATCCCTGGCCACAGCTGGATGCTGGATCTC NGG 10 8 17 8 24 0 100 5 10
    240605161734_g15u GCTAAAAATTCTGCCTCTCTGTCACTGTGCTG(+CAGTGT)GATCCGTCTAAAGTGGGACAGTTCAATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605161733_bigm TTTCAGACCATTGAACTGTCCCACTTTAGACG(+CAGTGT)GATCCAGCACAGTGACAGAGAGGCAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240605161731_ie5f ACCCAGTCTAAGCCCAGAGCTTGTTTGGGAGG(+CAGTGT)GATCCTCTCCTTCCTCCTGCTTGATCCTGTA NGG 10 8 17 8 24 0 100 5 10