Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161814_cu1x AAAGCTGGAGAAACTACTGCTTTAGAGAGCAG(+CAGTGT)GTACCACAGAAGGCAAGAAAAATTACAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605161812_jxxn TGTCAGTTTTTCTCTTTAGTGAAATGATACAG(+CAGTGT)GTACCAGCAAAGTGACAATATGTCCTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240605161811_nj05 TGGACATCAAAGGACATATTGTCACTTTGCTG(+CAGTGT)GTACCTGTATCATTTCACTAAAGAGAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605161809_37n2 AGTCCTCTAGTCTTGGCTGTGGGGGCTTCCAG(+CAGTGT)GTACCACTTGGGGAGAGGACTCAAGTAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605161808_mjji CACTTGTCTTACTTGAGTCCTCTCCCCAAGTG(+CAGTGT)GTACCTGGAAGCCCCCACAGCCAAGACTAGA NGG 10 8 17 8 24 0 100 5 10
    240605161807_a7ch GGGTTGGGAACAAGGGGGTGGGGTCATCAGAG(+CAGTGT)GTACCTGTTACTTTAGGGACCCTCTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605161805_uh98 CTCTCAGACAGAGAGGGTCCCTAAAGTAACAG(+CAGTGT)GTACCTCTGATGACCCCACCCCCTTGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605161804_qx4p CAGACTGGAGTTTTCTGCCTGTTTGTCTCTCG(+CAGTGT)GATCCTCAGAATAGCCGTGTGATGTGAGGTA NGG 10 8 17 8 24 0 100 5 10
    240605161803_ukrt CTGCCTACCTCACATCACACGGCTATTCTGAG(+CAGTGT)GATCCGAGAGACAAACAGGCAGAAAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240605161801_ynie CTCATGACTTTATATATTCAAAGCTTTTAATG(+CAGTGT)GTACCTGTTACACAGCAAGTACTCTGTGAAT NGG 10 8 17 8 24 0 100 5 10