Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161714_lznj AAGGAGAGCAAGCCCTGGGCAGAAAAGGCAAG(+CAGTGT)GTACCCAGCACCCTGACACCACACCACACCT NGG 10 8 17 8 24 0 100 5 10
    240605161712_vxba CAGAGCCTGGAGTCTGCGCTAATGAGACCATG(+CAGTGT)GATCCAGATTGCCCTAAGATGAAGATCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605161710_uqqk TAGCAGCAAGATCTTCATCTTAGGGCAATCTG(+CAGTGT)GATCCATGGTCTCATTAGCGCAGACTCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605161708_ph81 TCCTCCCCTCAGATGTGAGCACACCCTATGGG(+CAGTGT)GTACCCATCAACCCCTTGCCTCTGGGCCCCA NGG 10 8 17 8 24 0 100 5 10
    240605161706_ho9d GGATGTGGGGCCCAGAGGCAAGGGGTTGATGG(+CAGTGT)GTACCCCATAGGGTGTGCTCACATCTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605161704_4hqr TGTGGCTCACAGCAGCACTGAGTGGCCTTTGG(+CAGTGT)GTACCATGTGGCAGGCACACTGTCAGATGAG NGG 10 8 17 8 24 0 100 5 10
    240605161703_w0gc TTGTGCTCATCTGACAGTGTGCCTGCCACATG(+CAGTGT)GTACCCAAAGGCCACTCAGTGCTGCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605161701_jtej TCCCTGCAAGGCCAGCCCTGGGACAGTGCCTG(+CAGTGT)GTACCCGAGAAACAGTTATACGGGTGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605161659_h1gq CAGCGCAGGACACCCGTATAACTGTTTCTCGG(+CAGTGT)GTACCAGGCACTGTCCCAGGGCTGGCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605161656_ev3a AGATGGAGAGGACCTCAAGAGTCTGACCCTTG(+CAGTGT)GTACCATCCGATACTTAACTCCCTCGGTCCT NGG 10 8 17 8 24 0 100 5 10