Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161616_67bl GAGGAGGAGGTCTCTGGTCTAACCTCAGGCAG(+CAGTGT)GATCCAGGCTGCTCAGGCAGCTGCAACAGTA NGG 10 8 17 8 24 0 100 5 10
    240605161614_gdg5 ACCCTAAAGTTTCTAGAAGGAGGCAGGGAAGG(+CAGTGT)GTACCCCAGGCTCCCCATCCCCAGGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605161611_cgwj GGACTCAGCTCCCTGGGGATGGGGAGCCTGGG(+CAGTGT)GTACCCTTCCCTGCCTCCTTCTAGAAACTTT NGG 10 8 17 8 24 0 100 5 10
    240605161610_s9fi AAACAGCTTCATGTTTGCCCCATTTCTGTCAG(+CAGTGT)GATCCCCCAACCATGGGGGGCACTTGCACAA NGG 10 8 17 8 24 0 100 5 10
    240605161608_cx0k TATTTTTGTGCAAGTGCCCCCCATGGTTGGGG(+CAGTGT)GATCCTGACAGAAATGGGGCAAACATGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605161606_zrv5 GCCCTTAGCATGACGGGTTCTGCCCTTTGTGG(+CAGTGT)GATCCGGTAAGGAAACTGAGAGGTTCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605161604_szfp TTGAAACAAGGAACCTCTCAGTTTCCTTACCG(+CAGTGT)GATCCCACAAAGGGCAGAACCCGTCATGCTA NGG 10 8 17 8 24 0 100 5 10
    240605161602_opw8 ATTATAGGACAGAAAGTGCTGGGGTTGGGGAG(+CAGTGT)GTACCAAGAGGGTGAGTAGTAGGGGGAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605161601_tv0k GGGGAGTCTTCCCCCTACTACTCACCCTCTTG(+CAGTGT)GTACCTCCCCAACCCCAGCACTTTCTGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605161559_64u7 ATTGTTCAGTTCTGTGTGTGAGGGTAGGCAGG(+CAGTGT)GTACCTGCCCCATGTCAGCCGCCCTGATGAG NGG 10 8 17 8 24 0 100 5 10