Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161505_jmzq ATACACACACCGGAACGTGAAAACTCCCATAG(+CAGTGT)GTACCAGGCCGGCTGGCACAGCATGGGTCTT NGG 10 8 17 8 24 0 100 5 10
    240605161502_bti1 ATCGGGGTAAGAGGGCCATTAAGGAGGCCGAG(+CAGTGT)GTACCCTTTCTATGTTCCAAGCAGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605161500_qr8x CCCACCTCTCTCCTGCTTGGAACATAGAAAGG(+CAGTGT)GTACCTCGGCCTCCTTAATGGCCCTCTTACC NGG 10 8 17 8 24 0 100 5 10
    240605161459_suat AGAAGTCCCTGACAAGATGGTGTTTGGCTTGG(+CAGTGT)GATCCAGAGATGTCCAAATCCTGTCTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240605161458_8fh9 GCCACGTGGAAGACAGGATTTGGACATCTCTG(+CAGTGT)GATCCCAAGCCAAACACCATCTTGTCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605161456_khmn CTCCAGACCCATTTTTAGATAAGGGATTCAAG(+CAGTGT)GATCCAAGAAGACTTGTCCTGAATTCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605161455_uk80 GCTAGCTCAGGAATTCAGGACAAGTCTTCTTG(+CAGTGT)GATCCTTGAATCCCTTATCTAAAAATGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605161454_78hs TGACCATTTCTGTAATTGGTACTGTCTGGCAG(+CAGTGT)GTACCATGTGTGGCCTGGCTGTCCTGGAACA NGG 10 8 17 8 24 0 100 5 10
    240605161452_bsvl TGCTGTGTTCCAGGACAGCCAGGCCACACATG(+CAGTGT)GTACCTGCCAGACAGTACCAATTACAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605161451_tczg GGATGGACACAGGTCCCCCAGGGCCTAGGATG(+CAGTGT)GTACCTTGAGAAGACCAGCCTCATCATCCAT NGG 10 8 17 8 24 0 100 5 10