Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161542_dv06 TTCCTCTTTGTGGAGCCTGCTGTGTCCCTCTG(+CAGTGT)GATCCCTGTCCATTCACTCCTCACAGCTTAT NGG 10 8 17 8 24 0 100 5 10
    240605161540_7p67 GGAGAAACTGGGTTAAGGCCAGAGAAGTGCCG(+CAGTGT)GATCCTGCCCCAGATTTTGACTTTAGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605161538_bfm3 CATGGGAGCTCTAAAGTCAAAATCTGGGGCAG(+CAGTGT)GATCCGGCACTTCTCTGGCCTTAACCCAGTT NGG 10 8 17 8 24 0 100 5 10
    240605161537_svn0 GCCCGAGGTTCTGGTGGCCCAGCAACTGCAGG(+CAGTGT)GATCCATAAATCCAGAGGCCAGGATTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605161535_ahu9 ACTGAGCCTGAATCCTGGCCTCTGGATTTATG(+CAGTGT)GATCCCTGCAGTTGCTGGGCCACCAGAACCT NGG 10 8 17 8 24 0 100 5 10
    240605161534_uh2c ACAAGGGGAAGGAAGCCCAGGTAGAGGGTATG(+CAGTGT)GATCCCTCGAACCTGTCAGCAGACGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240605161532_crlk ACCGCGTGTTCCGTCTGCTGACAGGTTCGAGG(+CAGTGT)GATCCATACCCTCTACCTGGGCTTCCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605161531_c83o ATTCCTGGCCCTAGAAAGGAGGGTCTGCCGAG(+CAGTGT)GTACCAGGGGCCTGGCCTCACTGGCTGGACC NGG 10 8 17 8 24 0 100 5 10
    240605161527_7fhh GGTGTGGTCCAGCCAGTGAGGCCAGGCCCCTG(+CAGTGT)GTACCTCGGCAGACCCTCCTTTCTAGGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605161525_10ss GCAGTGGGGGCTTTCTCAGGAGAGATGCCAGG(+CAGTGT)GATCCCCAGGGACTGCACACCCCACTATCTC NGG 10 8 17 8 24 0 100 5 10