Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605161638_fyb5 AGACAGGGCCACAGAACTGCAATGACACAGTG(+CAGTGT)GTACCCAATGTTTATATAACTTCCCTGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605161635_tg0l GCAGGGCAATGGCACGGCTGTGAGGTTGCGGG(+CAGTGT)GTACCCAGGAGTTTCTTAGCAAAGATGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605161633_ukh6 GTCTAGAAGCATCTTTGCTAAGAAACTCCTGG(+CAGTGT)GTACCCCGCAACCTCACAGCCGTGCCATTGC NGG 10 8 17 8 24 0 100 5 10
    240605161630_8pma GACGTTCAGGGTTTGCCCTCTTCTCCTCCTGG(+CAGTGT)GTACCTTCCACTGCGCTCTTCACCCCCGCTG NGG 10 8 17 8 24 0 100 5 10
    240605161628_cwft GCCTTCAGCGGGGGTGAAGAGCGCAGTGGAAG(+CAGTGT)GTACCCAGGAGGAGAAGAGGGCAAACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605161626_dz8d TCCTTGCATGTTTATGCTCTTATGTATTCCTG(+CAGTGT)GTACCCAGGGAGGCCAGAAGAGGGCTGATTC NGG 10 8 17 8 24 0 100 5 10
    240605161623_gpkc CCAGGGAATCAGCCCTCTTCTGGCCTCCCTGG(+CAGTGT)GTACCAGGAATACATAAGAGCATAAACATGC NGG 10 8 17 8 24 0 100 5 10
    240605161621_3sjv TGCTGCCGTGTATGTGCTGGGAATTGAACCAG(+CAGTGT)GATCCTCTGGATTTGCAGCTAGAACTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605161619_zfpc CATGGTTAAAAGTTCTAGCTGCAAATCCAGAG(+CAGTGT)GATCCTGGTTCAATTCCCAGCACATACACGG NGG 10 8 17 8 24 0 100 5 10
    240605161617_pv30 CAGCTTACTGTTGCAGCTGCCTGAGCAGCCTG(+CAGTGT)GATCCTGCCTGAGGTTAGACCAGAGACCTCC NGG 10 8 17 8 24 0 100 5 10