Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162012_w0ca GGGCAATTGTGCTGGAAGACTGTAGAGGTTGG(+CAGTGT)GTACCCCTTCAGTTGCTGATTACTACATAAG NGG 10 8 17 8 24 0 100 5 10
    240605162010_dfvn TCAAAGCCTTCTGAGACCCCATCCCACACGAG(+CAGTGT)GTACCGTGAAGGGGCACTAAAGTGCATTCTG NGG 10 8 17 8 24 0 100 5 10
    240605162009_llpb TCCTTCAGAATGCACTTTAGTGCCCCTTCACG(+CAGTGT)GTACCTCGTGTGGGATGGGGTCTCAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605162007_66we CTTGAGCATCATACTGCTTTTAAATCCATGGG(+CAGTGT)GATCCTGGAAGAGCTCCCCTACAGATACTGA NGG 10 8 17 8 24 0 100 5 10
    240605162005_khoh GCCCCTCAGTATCTGTAGGGGAGCTCTTCCAG(+CAGTGT)GATCCCCATGGATTTAAAAGCAGTATGATGC NGG 10 8 17 8 24 0 100 5 10
    240605162004_v8no AATAAAGCTGGACTGAAAGAAACATCTCTGAG(+CAGTGT)GATCCAGGAAGTGGACTGTCATTCACCAAAT NGG 10 8 17 8 24 0 100 5 10
    240605162002_47ii AGTTGATTTGGTGAATGACAGTCCACTTCCTG(+CAGTGT)GATCCTCAGAGATGTTTCTTTCAGTCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605162000_g64c TAATTTTTTGTAACTTTCCACTACTCTACCTG(+CAGTGT)GTACCATATCTGTGGATTTTGGCCAATATTA NGG 10 8 17 8 24 0 100 5 10
    240605161959_xala AGTTTTAATATTGGCCAAAATCCACAGATATG(+CAGTGT)GTACCAGGTAGAGTAGTGGAAAGTTACAAAA NGG 10 8 17 8 24 0 100 5 10
    240605161957_3n6b TGTGGCAGGGTGGATAATGCGTGAGGACTTGG(+CAGTGT)GATCCTGATGGACTTTTAATTGTACCCACAA NGG 10 8 17 8 24 0 100 5 10