Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162113_6kff CGCTGACAGTACCATGCAGCTGACTGGACACG(+CAGTGT)GTACCGCCCGTTCTCCTGGGCCTCGGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605162112_ero2 TGAGCTAATTGTGTGTGTGCCTTTCATCTGAG(+CAGTGT)GATCCGAGAGAGCTCCTGGGCAGGTACGAGC NGG 10 8 17 8 24 0 100 5 10
    240605162111_4kqz CGCAGGCTCGTACCTGCCCAGGAGCTCTCTCG(+CAGTGT)GATCCTCAGATGAAAGGCACACACACAATTA NGG 10 8 17 8 24 0 100 5 10
    240605162109_on9d TACCCTAGTTCTATAGAGCAGCTTGGACACTG(+CAGTGT)GTACCTTGGTCTTCTGCCTTGAAGCTGATTT NGG 10 8 17 8 24 0 100 5 10
    240605162108_pmbk CATGGAAATCAGCTTCAAGGCAGAAGACCAAG(+CAGTGT)GTACCAGTGTCCAAGCTGCTCTATAGAACTA NGG 10 8 17 8 24 0 100 5 10
    240605162106_myin GTTAGGATTGCAACATGCCTCACTATCTCAAG(+CAGTGT)GTACCTAGATTTTTATTTGCTAAATCTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605162105_vbeu TTGATATTTAGATTTAGCAAATAAAAATCTAG(+CAGTGT)GTACCTTGAGATAGTGAGGCATGTTGCAATC NGG 10 8 17 8 24 0 100 5 10
    240605162103_9o20 GTACACTCTTCAGGACCACCTGTCTAGGGCTG(+CAGTGT)GTACCAGCCACAAGGGCTGCTTCCTCCCACA NGG 10 8 17 8 24 0 100 5 10
    240605162102_je84 ATTGATGTGGGAGGAAGCAGCCCTTGTGGCTG(+CAGTGT)GTACCAGCCCTAGACAGGTGGTCCTGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605162100_d8ma AATCCTTTGAGTGAACCTAGTGTTTCCGGCTG(+CAGTGT)GATCCTGAGAGATACAAATGGAAAAATACTG NGG 10 8 17 8 24 0 100 5 10