Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162228_1qik TGAGCCACAAAGTTCCCACGAGTTATCATTAG(+CAGTGT)GATCCTGGCCACTGTTTTTGCCATGGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605162226_j88d GGAAGGAAAACCATGGCAAAAACAGTGGCCAG(+CAGTGT)GATCCTAATGATAACTCGTGGGAACTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605162225_boec AAATCACTGACAATGCCTATATTAGTTAATGG(+CAGTGT)GATCCATCTCAACCCTAAAAAGAAGGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605162224_akkl ATTCATTTTTCCTTCTTTTTAGGGTTGAGATG(+CAGTGT)GATCCCATTAACTAATATAGGCATTGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605162222_9g6d TTGCCTGAACTGATATCTTGGTTTTTCATCCG(+CAGTGT)GATCCAGTCAGGACAGAGACATCAGAAACTA NGG 10 8 17 8 24 0 100 5 10
    240605162220_tj6h TGTATTAGTTTCTGATGTCTCTGTCCTGACTG(+CAGTGT)GATCCGGATGAAAAACCAAGATATCAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240605162219_mzt5 AAACCCAAAGCATACGCAGGAGTCAGTTCCTG(+CAGTGT)GTACCGTAGAGTGAGAAAGACACCTGTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605162217_c8lj CTTCCCTGCACAGGTGTCTTTCTCACTCTACG(+CAGTGT)GTACCAGGAACTGACTCCTGCGTATGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605162216_yh6a CAGGGTAGGTGTAGTTCTGAGAGTTGTGAGAG(+CAGTGT)GATCCAAAGATCACTTGGGCAGGTTTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240605162215_o3sw AAATCTACTCAAACCTGCCCAAGTGATCTTTG(+CAGTGT)GATCCTCTCACAACTCTCAGAACTACACCTA NGG 10 8 17 8 24 0 100 5 10