Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605162243_fgv1 CTGTGCCCCACAGAGGCCATAAGAGGGCCTTG(+CAGTGT)GATCCCTATAACTAATTAGACTGTTTTAAGT NGG 10 8 17 8 24 0 100 5 10
    240605162241_gjh8 CAGTAACTTAAAACAGTCTAATTAGTTATAGG(+CAGTGT)GATCCAAGGCCCTCTTATGGCCTCTGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605162240_h7zl TTCCCAGTTGCTTTGTAAACTGCATTATAATG(+CAGTGT)GATCCATTTAAGGAAAATGATCTCTTAAATA NGG 10 8 17 8 24 0 100 5 10
    240605162239_rfja GGTTTTATTTAAGAGATCATTTTCCTTAAATG(+CAGTGT)GATCCATTATAATGCAGTTTACAAAGCAACT NGG 10 8 17 8 24 0 100 5 10
    240605162237_m3yn GCCTTCAAGATATATCCAAACTAGGTACAAAG(+CAGTGT)GATCCACAAATATCAACAATTTGTAACCAAA NGG 10 8 17 8 24 0 100 5 10
    240605162236_6o90 AGAATTTTGGTTACAAATTGTTGATATTTGTG(+CAGTGT)GATCCTTTGTACCTAGTTTGGATATATCTTG NGG 10 8 17 8 24 0 100 5 10
    240605162235_l5gu CAAGTTACACACTGGAAACAAAGCAGAACATG(+CAGTGT)GTACCTGCTTTTACTAACTCAAAACAAGTTA NGG 10 8 17 8 24 0 100 5 10
    240605162234_qdh8 TTTGCTAACTTGTTTTGAGTTAGTAAAAGCAG(+CAGTGT)GTACCATGTTCTGCTTTGTTTCCAGTGTGTA NGG 10 8 17 8 24 0 100 5 10
    240605162231_d3qv TCAATCATTTAATTGACCGCAGTAGTCGCCGG(+CAGTGT)GTACCCGGCACTAAGCATGCACTACATCACA NGG 10 8 17 8 24 0 100 5 10
    240605162229_i0we GCTTCTGTGATGTAGTGCATGCTTAGTGCCGG(+CAGTGT)GTACCCGGCGACTACTGCGGTCAATTAAATG NGG 10 8 17 8 24 0 100 5 10