Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155617_9hl9 TCCCCAGAGACTTAAGTCCACACCAGGGAAAG(+CAGTGT)GATCCATGCCACGAGGGGCAGAGCAGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605155615_zzuv CAGGAGGCTGCTATGGGCTAAAATTTTCCTAG(+CAGTGT)GTACCACCTAGAGGAGATGGAGCCGACCGAG NGG 10 8 17 8 24 0 100 5 10
    240605155614_5blw CTTCCCTCGGTCGGCTCCATCTCCTCTAGGTG(+CAGTGT)GTACCTAGGAAAATTTTAGCCCATAGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605155612_t0uj CAGGAATGCCATCTTCTGCCTTGGTCTGGAAG(+CAGTGT)GTACCCATGGCTACTTGCATCATTTTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605155610_97uj ACAAGGCTGGAAAATGATGCAAGTAGCCATGG(+CAGTGT)GTACCTTCCAGACCAAGGCAGAAGATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605155607_fk0n AAAAAGACCTGTGGAGATCTCAGAATCCACCG(+CAGTGT)GTACCATACTGCAAGGGATACTGACCTTAAG NGG 10 8 17 8 24 0 100 5 10
    240605155606_nd3p AGGAACTTAAGGTCAGTATCCCTTGCAGTATG(+CAGTGT)GTACCGGTGGATTCTGAGATCTCCACAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605155604_fvfo AGCCGAGACCCCCTCCCCTGCAGTCAGTGAGG(+CAGTGT)GATCCGTGGAGGTCCTGAACAAGTGTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605155602_zni4 GAAGAAGACAACACTTGTTCAGGACCTCCACG(+CAGTGT)GATCCCTCACTGACTGCAGGGGAGGGGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605155600_sz49 TTGCATTTCATGCATCTCCCCTGATCTCTGGG(+CAGTGT)GTACCTGCCAACCTCCCTTCGACAAGGGCAG NGG 10 8 17 8 24 0 100 5 10