Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155514_cpi2 GAGTTCAGTTTCCCAGGGGAACAGAAGGACTG(+CAGTGT)GTACCCAGCTGAAGCAATGAGACAGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605155512_fbm1 TGCAATCCCAGGGCTGGGAGATGGAGCCAGAG(+CAGTGT)GATCCCTGGGACTTGCTGGCCAGCCAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240605155509_rq6o TTAACTAGCCTGGCTGGCCAGCAAGTCCCAGG(+CAGTGT)GATCCTCTGGCTCCATCTCCCAGCCCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605155507_s84g GTGGTATTGCATTTAAATGGACAGGACACGGG(+CAGTGT)GTACCAAATCCTACAATGTGTCAGTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605155505_igj5 AATTGCTCCAAACTGACACATTGTAGGATTTG(+CAGTGT)GTACCCCGTGTCCTGTCCATTTAAATGCAAT NGG 10 8 17 8 24 0 100 5 10
    240605155503_4pbl TGCTCTTTATTCTCAGGGCCCGCACACATCCG(+CAGTGT)GTACCTTCATAAATGCAGTGTCACTGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605155502_mmdd TCCATTTCTTCAGTGACACTGCATTTATGAAG(+CAGTGT)GTACCGGATGTGTGCGGGCCCTGAGAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605155500_ilsm AGCACACTGCTATGTGTGGCCTTGATGCTGGG(+CAGTGT)GATCCTAACTCAGGTTCTCGTGCTTTACCGA NGG 10 8 17 8 24 0 100 5 10
    240605155459_lzew CCCAGTCGGTAAAGCACGAGAACCTGAGTTAG(+CAGTGT)GATCCCCAGCATCAAGGCCACACATAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605155457_2g83 AGAAACCAGGGCCAGCGTTGGGGGTGCAGGAG(+CAGTGT)GTACCCAGAGAAGCAAAAACATTCAAAGGAA NGG 10 8 17 8 24 0 100 5 10