Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155407_hwch ATCCCTGGTAAGAGAGGAGCTCCTGGTGACTG(+CAGTGT)GATCCCTTTGATACCCAGGACTATGAATCGA NGG 10 8 17 8 24 0 100 5 10
    240605155406_fv1i GGGGACAAGGTCACCTGAACTTAGCTCTCAAG(+CAGTGT)GATCCACACAAACTCAAAGCCTTAGTGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605155405_piod GGCCACTACCACTAAGGCTTTGAGTTTGTGTG(+CAGTGT)GATCCTTGAGAGCTAAGTTCAGGTGACCTTG NGG 10 8 17 8 24 0 100 5 10
    240605155403_x8np ACACAGCAATGGGAATAGCCACCGAGTTTAAG(+CAGTGT)GTACCAGGTGGCAACAAACCAATAATAACTT NGG 10 8 17 8 24 0 100 5 10
    240605155402_blov TAGTTAAGTTATTATTGGTTTGTTGCCACCTG(+CAGTGT)GTACCTTAAACTCGGTGGCTATTCCCATTGC NGG 10 8 17 8 24 0 100 5 10
    240605155400_0zcv GTGGGCATATGTGGGTGCCAGGCTGGAACAAG(+CAGTGT)GTACCACTTTTAGAGTGAGATGTGACATTGT NGG 10 8 17 8 24 0 100 5 10
    240605155359_x932 ACTCAACAATGTCACATCTCACTCTAAAAGTG(+CAGTGT)GTACCTTGTTCCAGCCTGGCACCCACATATG NGG 10 8 17 8 24 0 100 5 10
    240605155357_qtgp CAACTTAGGGGGTGAGCAGGTGTTGGTGATGG(+CAGTGT)GATCCCTGTGATGAGATGGGGGTGACACTGC NGG 10 8 17 8 24 0 100 5 10
    240605155356_kg76 AATGTGCAGTGTCACCCCCATCTCATCACAGG(+CAGTGT)GATCCCATCACCAACACCTGCTCACCCCCTA NGG 10 8 17 8 24 0 100 5 10
    240605155354_778t TCGAATATTTAGAAGTGTAAAACATAAACCAG(+CAGTGT)GATCCTGCCCCCCACATGTCCAGGACGATGA NGG 10 8 17 8 24 0 100 5 10