Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605155308_dbbb GGAAACACCGGAGCATAGACATCGGGCCTGTG(+CAGTGT)GATCCAAGAAGAGACCTAGAATGGTTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605155307_wwyy AATCCCAGGGAACCGTTCTAGGTCTCTTCTTG(+CAGTGT)GATCCACAGGCCCGATGTCTATGCCCCGGTG NGG 10 8 17 8 24 0 100 5 10
    240605155305_ifye GGGAACACCGGGGCATAGACATCGGGCCTGTG(+CAGTGT)GATCCAAGAAGAGACCTAGAACGGTTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605155304_zu23 AATCCCAGGGAACCGTTCTAGGTCTCTTCTTG(+CAGTGT)GATCCACAGGCCCGATGTCTATGCCCCGGTG NGG 10 8 17 8 24 0 100 5 10
    240605155302_6q7f GGGAACACCGGGGCATAGACATCGGGCCTGTG(+CAGTGT)GATCCAAGAAGAGACCTAGAACGGTTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605155300_0m78 TAGATGGGCAGCAATGGGCAGTTGCAACCAAG(+CAGTGT)GTACCCAGCAGTGTCTTCTACATCACCTATA NGG 10 8 17 8 24 0 100 5 10
    240605155258_9aaw CTGGATATAGGTGATGTAGAAGACACTGCTGG(+CAGTGT)GTACCTTGGTTGCAACTGCCCATTGCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605155257_1o7c GACAGGATGGGCTCCACACGTACCTGTGTTGG(+CAGTGT)GATCCCCACATTCGCTGTAACCTATCTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605155255_limn GTGTGCTACAGATAGGTTACAGCGAATGTGGG(+CAGTGT)GATCCCAACACAGGTACGTGTGGAGCCCATC NGG 10 8 17 8 24 0 100 5 10
    240605155253_u91d AAGAGCACCACCCAATCACAAGCCTGGGGTTG(+CAGTGT)GATCCATGCCAGCCAAGGACTATGATTTCCA NGG 10 8 17 8 24 0 100 5 10